Frequently Asked Questions

Portable Raman FAQs

 

Q - In taking Raman spectra, is it most common to do so and display them with the option dark subtracted chosen?
A - Yes. For the best results it is recommended to use Dark Subtract. Make sure that your saved Dark Scan matches the integration time of your acquisition scan.

 

Q - What are the typical conditions (laser power and integration time) for
acquiring Raman spectra of solutions using the cuvette holder?

A - For a cuvette with acetone, you may use full power with a 3-5 second integration time.

 

Q - When positioning the Raman probe in the cuvette holder how close should the probe be to the cuvette surface?
A - Insert the probe all the way in, there is a stopper inside the BCR100A for ideal positioning.

 

532nm Laser/System:
Q - If the interlock is pulled out and the laser is turned off, how much time is needed for the laser to stabilize when the laser turns back on?
A - Approximately 30 seconds is needed for the laser to stabilize after the laser starts emitting energy.
*Some older generation probes may not block the entire laser signal. A simple test can be
done by increasing the exposure time, leaving the laser on, switching the shutter on the probe
to off and then taking a scan to see if any laser signal is present.

 

785nm Laser/System:
Q - If the interlock is pulled out and the laser is turned off, how much time is needed for the laser to stabilize after the laser turns back on?
A - Approximately 2-5 seconds is needed to for the laser to stabilize after the laser starts emitting energy.

 

Q - Can my Raman Probe be used to take a dark scan by simply closing the shutter on the probe?
A - Yes, the dark scan can be taken by closing the shutter on the probe. It is also recommended to turn off or otherwise block ambient light when measuring Raman signals.
*Some older generation probes may not block the entire laser signal. A simple test can be
done by increasing the exposure time, leaving the laser on, switching the shutter on the probe
to off and then taking a scan to see if any laser signal is present.

 

Q - What is the recommended way to measure a solid sample?
A - In order to measure a solid sample, place the Raman probe shaft with the distance regulator directly in contact with a sample to collect the Raman spectrum. The distance regulator is designed for optimal focus and to also exclude extraneous light from the measurement.

 

Q - Can I measure liquids directly?
A - An immersion probe shaft is needed to make measurements in a liquid sample. Liquids can also be measured through a glass vial or a cuvette. We offer a liquid vial holder (NR-LVH) and a cuvette holder (BCR100A) designed for use with the i-Raman® series lab probes.

 

Q - Can I do Raman microscopy with a portable Raman?
A - The i-Raman® series instruments can be used with the BAC151 series video microscopes which have an integrated camera and are readily interfaced with the fiber-optically coupled probe. This setup provides precision microsampling through camera monitoring and imaging details.

 

Q - Can I use my i-Raman® Plus for fieldwork?
A - The i-Raman series instruments are portable, with the i-Raman Plus weighing less than 7 lbs. The system is battery operable and can be used with a range of sampling accessories including a tripod for use in the field.

 

Q - Does BWSpec® software provide tools to optimize data collection?
A - Both BWSpec® and BWID® software used with the i-Raman series instruments have auto-integration time functions that will optimize the acquisition time to give optimal signal intensity.

 

Q - What is the recommended way of collecting the Raman spectrum of a delicate or heat sensitive sample?
A - The laser power can be adjusted down to 1% laser power (< 5mW). Using low laser power is recommended for measurements of delicate samples to minimize the risk of sample damage.